Detection of Antibodies to Melanocytes in Vitiligo by Specific Immunoprecipitation

Antibodies to normal human melanocytes in vitiligo

Most patients with active vitiligo (82% of 61) have antibodies to antigens of normal human melanocytes that can be detected by specific immunoprecipitation of radioiodinated, detergent-soluble, melanocyte macromolecules. Such antibodies were present in only 12% of patients with melanoma and in none of 35 patients with nonpigmentary skin diseases. The antibodies were directed to a common antigen...

Detection of auto antibodies and transplantation of cultured autologous melanocytes for the treatment of vitiligo

The aim of the present study was to establish an immunofluorescence method of antibody detection to identify melanocytes in the serum of vitiligo patients. Furthermore, we aimed to establish a method for the culture and proliferation of autologous pure melanocytes and to observe the effect of their transplantation for the treatment of vitiligo. Suspension of epidermal cells with melanocytes was...

Relationship between the serum TGF beta 1 level and anti organ-specific antibodies in vitiligo patients

Background: To our knowledge, there is a hypothesis regardingthe association of vitiligo with other autoimmune disorders.Organ-specific autoantibodies are found more frequently in theserum of vitiligo patients. Recent studies have demonstratedthe role of alterations in serum transforming growth factor-beta(TGF-beta) released from regulatory T cells in the pathogenesisof depigmentation observed ...

extremal region detection guided by maxima of gradient magnitude

a problem of computer vision applications is to detect regions of interest under dif- ferent imaging conditions. the state-of-the-art maximally stable extremal regions (mser) detects affine covariant regions by applying all possible thresholds on the input image, and through three main steps including: 1) making a component tree of extremal regions’ evolution (enumeration), 2) obtaining region ...

ifn-γ promotes apoptosis of melanocytes in vitiligo